유리규산에 의한 폐장내 IL-1β, IL-6, TNF-α, TGF-β의 발현
- Alternative Title
- Silica induced Expression of IL-1beta, IL-6, TNF-beta, TGF-alpha, in the Experimental Murine Lung Fibrosis
- Abstract
- Background: Silica-induced lung diseases is characterized by the accumulation of inflammatory cells at early stage and fibrosis in pulmonary parenchyma and interstitium at late stage. As a consequence of inflammation, silicosis is accompanied with the expansion of interstitial collagen and the formation of fibrotic nodule. In this process, several kinds of lung cells produce cytokines which can amplify and modulate pulmonary fibrosis. The alveolar macrophage is a potent source of proflammatory cytokines and growth factor. But in the process of silicotic inflammation and fibrosis, there are many changes of the kinetics in cytokine network. And the sources of cytokines in each phase are not well known. Method: 2.5 mg of silica was instillated into the lung of C57BL/6J mice. After intratracheal instillation of silica, the lungs were removed for imunohistochemical stain at 1, 2, 7 day, 2, 4, 8, 12 week, respectively. We investigated the expression of IL-1$\beta$, IL-6, TNF-$\alpha$ and TGF-$\beta$ in lung tissue. Results: 1) The expression of IL-6 increased from 1 day after exposure to 8 weeks in vascular endothelium. Also peribronchial area were stained for IL-6 from 7 days and reached the peak level for 4 weeks. 2) The IL-1 $\beta$ was expressed weakly at the alveolar and peribronchial area through 12 weeks. 3) The TNF-$\alpha$ expressed strongly at alveolar and bronchial epithelia during early stage and maintained for 12 weeks. 4) TGF-$\beta$ was expressed strongly at bronchial epithelia and peribronchial area after 1 week and the strongest at 8 weeks. Conclusion: The results above suggests IL-6, TNF-$\alpha$ appear to be a early inflammatory response in silica induced lung fibrosis and TGF-$\beta$ play a major role in the maintenance and modulation of fibrosis in lung tissue. And the regulation of TNF-$\alpha$ production will be a key role in modultion of silica-induced fibrosis.
- All Author(s)
- 기신영
; 박성우
; 이명란
; 김은영
; 어수택
; 김용훈
; 박춘식
; 이희발
- Issued Date
- 1998
- Type
- Article
- Keyword
- silica; fibrosis; macrophage; immunohistochemical stain; TNF-α; TGF-β
- Publisher
- 대한결핵및호흡기학회
- ISSN
- 0378-0066
; 1738-3536
; 2005-6184
- Citation Title
- 결핵 및 호흡기질환
Tuberculosis and Respiratory Disease
- Citation Volume
- 45
- Citation Number
- 4
- Citation Start Page
- 835
- Citation End Page
- 845
- Language(ISO)
- kor
- DOI
- 10.4046/trd.1998.45.4.835
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/515
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