Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
- Abstract
- Objectives: The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
Materials and methods: Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
Results: The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
Conclusion: These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
- All Author(s)
- M. J. Ji
; B. K. Cho
; Y. S. Cho
; Y. J. Choi
; D. Kwon
; K. Shin
; J. Y. Lee
; C. Kang
; B. S. Yoon
- Issued Date
- 2013
- Type
- Article
- Keyword
- immunochromatographic assay; immunochromatography test; influenza; pdm09
- Publisher
- 질병관리본부
Korea Centers for Disease Control & Prevention
- ISSN
- 2210-9099
; 2233-6052
- Citation Title
- Osong public health and research perspectives
- Citation Volume
- 4
- Citation Number
- 6
- Citation Start Page
- 342
- Citation End Page
- 346
- Language(ISO)
- eng
- DOI
- 10.1016/j.phrp.2013.10.006
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2581
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