Complete sequence analysis and antiviral screening of medicinal plants for human coxsackievirus a16 isolated in Korea
- Abstract
- Objectives: Coxsackievirus A group 16 strain (CVA16) is one of the predominant causative agents of hand, foot, and mouth disease (HFMD).
Methods: Using a specimen from a male patient with HFMD, we isolated and performed sequencing of the Korean CVA16 strain and compared it with a G10 reference strain. Also, we were investigated the effects of medicinal plant extract on the cytopathic effects (CPE) by CPE reduction assay against Korean CVA16.
Results: Phylogenetic analysis showed that the Korean CVA16 isolate belonged to cluster B-1 and was closely related to the strain PM-15765-00 isolated in Malaysia in 2000. The Korean CVA16 isolate showed 73.2% nucleotide identity to the G10 prototype strain and 98.7% nucleotide identity to PM-15765-00. Next, we assessed whether the Korean CVA16 isolate could be used for in vitro screening of antiviral agents to treat HFMD infection. Vero cells infected with the Korean CVA16 isolate showed a cytopathic effect 2 days after the infection, and the treatment of cells with Cornus officinalis, Acer triflorum, Pulsatilla koreana, and Clematis heracleifolia var. davidiana Hemsl extracts exhibited strong antiviral activity against CVA16.
Conclusion: Collectively, our work provides potential candidates for the development of vaccine and novel drugs to treat the CVA16 strain isolated from a Korean patient.
- All Author(s)
- J. H. Song
; K. Park
; A. Shim
; B. E. Kwon
; J. H. Ahn
; Y. J. Choi
; J. K. Kim
; S. G. Yeo
; K. Yoon
; H. J. Ko
- Issued Date
- 2015
- Type
- Article
- Keyword
- HFMD; antiviral drug; coxsackievirus A16; medicinal plant
- Publisher
- 질병관리본부
Korea Centers for Disease Control & Prevention
- ISSN
- 2210-9099
; 2233-6052
- Citation Title
- Osong public health and research perspectives
- Citation Volume
- 6
- Citation Number
- 1
- Citation Start Page
- 52
- Citation End Page
- 58
- Language(ISO)
- eng
- DOI
- 10.1016/j.phrp.2014.12.004
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2580
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