The role of Wnt/β-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation
- Abstract
- To investigate the role of Wnt/β-catenin signaling pathway in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE) after laser photocoagulation. After differentiation of RPE cells from hiPSCs, laser photocoagulation was performed. Activation of Wnt/β-catenin signaling at days 1 and 5 after laser photocoagulation was evaluated by expression of β-catenin. Cell proliferation and alteration in cell-to-cell contact at day 5 after laser photocoagulation with or without Dickkopf-1 (Dkk-1) treatment were studied using ethynyl-2'-deoxyuridine (EdU) assay and zonula occludens-1 (ZO-1) expression analysis, respectively. The mRNA levels of Wnt genes at day 5 after laser photocoagulation were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Activation of Wnt/β-catenin signaling at days 1 and 5 after laser photocoagulation was confirmed by β-catenin accumulation in the cytoplasm and nucleus of hiPSC-RPE. Many EdU-positive cells also expressed β-catenin, and the number of EdU-positive cells was decreased at day 5 after laser photocoagulation after Dkk-1 treatment, indicating that Wnt/β-catenin signaling mediated hiPSC-RPE proliferation. ZO-1 expression was not decreased with Dkk-1 treatment at day 5 after laser photocoagulation, indicating that Wnt/β-catenin signaling mediated hiPSC-RPE restoration. At day 5, after laser photocoagulation, mRNA levels of Wnt2b, Wnt3, Wnt5a, Wnt7a, and Wnt10b were increased. Wnt/β-catenin signaling has a crucial role in restoration of hiPSC-RPE proliferation after laser photocoagulation. Manipulation of Wnt/β-catenin signaling while elucidating the underlying mechanisms of RPE restoration might have a therapeutic potential in retinal degenerative diseases.
- All Author(s)
- I. H. Cho
; S. J. Park
; S. H. Lee
; S. K. Nah
; H. Y. Park
; J. Y. Yang
; S. B. Madrakhimov
; J. Lyu
; T. K. Park
- Issued Date
- 2019
- Type
- Article
- Keyword
- Induced pluripotent stem cell; Laser photocoagulation; Retinal pigment epithelium; Wnt/β-catenin signaling pathway
- Publisher
- European Laser Association
- ISSN
- 0268-8921
; 1435-604X
- Citation Title
- Lasers in medical science
- Citation Volume
- 34
- Citation Number
- 3
- Citation Start Page
- 571
- Citation End Page
- 581
- Language(ISO)
- eng
- DOI
- 10.1007/s10103-018-2631-5
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2382
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