Oxidative Damage and Nuclear Factor Erythroid 2-Related Factor 2 Protein Expression in Normal Skin and Keloid Tissue
- Abstract
- BACKGROUND: Reactive oxygen species (ROS) play an important role in the induction of apoptosis under pathological conditions. Recently, a significant increase in ROS production and disrupted apoptosis mechanisms in keloids have been reported. Nuclear factor erythroid 2-related factor 2 (Nrf2) represents one of the most important cellular defense mechanisms against oxidative stress and is implicated in the regulation of apoptosis. Recently, it has been reported that Nrf2 upregulates Bcl-2, an anti-apoptotic protein. OBJECTIVE: To compare Nrf2 protein expression in normal skin tissues to keloid tissues. METHODS: ROS generation in keloid tissues was evaluated with OxyBlot analysis. Western blotting and/or immunohistochemical staining approaches were used to study expression of Nrf2 or Bcl-2 in keloid and normal skin tissues. Cellular fractionation was performed to examine subcellular distribution of Nrf2. Transfection of fibroblasts with Nrf2-specific small interfering RNA (siRNA) was conducted to understand the relationship between Nrf2 expression and apoptosis induction. RESULTS: Protein oxidation, a marker of oxidative stress, is increased in keloid tissues. Western blot analysis clearly showed that Nrf2 and Bcl-2 are downregulated in keloid tissues. Immunohistochemical staining of Nrf2 confirmed the results of the western blot analysis. Transfection of fibroblasts with the Nrf2-specific siRNA results in increased apoptosis and decreased cell viability. CONCLUSION: Collectively, our data indicate that Nrf2 expression is downregulated in keloid tissues, and that Nrf2 is involved in the development of apoptosis in Nrf2 siRNA-transfected fibroblasts. We propose that a defective antioxidant system and apoptotic dysregulation may participate in keloid pathogenesis.
- All Author(s)
- Y. J. Lee
; S. B. Kwon
; C. H. Kim
; H. D. Cho
; H. S. Nam
; S. H. Lee
; M. W. Lee
; D. H. Nam
; C. Y. Choi
; M. K. Cho
- Issued Date
- 2015
- Type
- Article
- Keyword
- Apoptosis; Bcl2; Keloid; NF-E2-related factor 2; Reactive oxygen species
- Publisher
- 대한피부과학회
- ISSN
- 1013-9087
- Citation Title
- Annals of Dermatology
- Citation Volume
- 27
- Citation Number
- 5
- Citation Start Page
- 507
- Citation End Page
- 516
- Language(ISO)
- eng
- DOI
- 10.5021/ad.2015.27.5.507
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/1374
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