Clinical Usefulness of a Cell-based Assay for Detecting Myelin Oligodendrocyte Glycoprotein Antibodies in Central Nervous System Inflammatory Disorders
- Abstract
- Background: The clinical implications of myelin oligodendrocyte glycoprotein autoantibodies (MOG-Abs) are increasing. Establishing MOG-Ab assays is essential for effectively treating patients with MOG-Abs. We established an in-house cell-based assay (CBA) to detect MOG-Abs to identify correlations with patients' clinical characteristics.
Methods: We established the CBA using HEK 293 cells transiently overexpressing full-length human MOG, tested it against 166 samples from a multicenter registry of central nervous system (CNS) inflammatory disorders, and compared the results with those of the Oxford MOG-Ab-based CBA and a commercial MOG-Ab CBA kit. We recruited additional patients with MOG-Abs and compared the clinical characteristics of MOG-Ab-associated disease (MOGAD) with those of neuromyelitis optica spectrum disorder (NMOSD).
Results: Of 166 samples tested, 10 tested positive for MOG-Abs, with optic neuritis (ON) being the most common manifestation (4/15, 26.7%). The in-house and Oxford MOG-Ab CBAs agreed for 164/166 (98.8%) samples (κ=0.883, P<0.001); two patients (2/166, 1.2%) were only positive in our in-house CBA, and the CBA scores of the two laboratories correlated well (r=0.663, P<0.001). The commercial MOG-Ab CBA kit showed one false-negative and three false-positive results. The clinical presentation at disease onset differed between MOGAD and NMOSD; ON was the most frequent manifestation in MOGAD, and transverse myelitis was most frequent in NMOSD.
Conclusions: The in-house CBA for MOG-Abs demonstrated reliable results and can potentially be used to evaluate CNS inflammatory disorders. A comprehensive, long-term study with a large patient population would clarify the clinical significance of MOG-Abs.
- All Author(s)
- Jin Myoung Seok
; Patrick Waters
; Mi Young Jeon
; Hye Lim Lee
; Seol-Hee Baek
; Jin-Sung Park
; Sa-Yoon Kang
; Ohyun Kwon
; Jeeyoung Oh
; Byung-Jo Kim
; Kyung-Ah Park
; Sei Yeul Oh
; Byoung Joon Kim
; Ju-Hong Min
- Issued Date
- 2024
- Type
- Article
- Keyword
- Autoantibody; Central nervous system disease; Immunoassay; Myelin oligodendrocyte glycoprotein
- Publisher
- 대한진단검사의학회
- ISSN
- 2234-3806
; 2234-3814
- Citation Title
- Annals of laboratory medicine
- Citation Volume
- 44
- Citation Number
- 1
- Citation Start Page
- 56
- Citation End Page
- 63
- Language(ISO)
- eng
- DOI
- 10.3343/alm.2024.44.1.56
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/1270
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.